Sequencing of the genomic RNA of
Grapevine leafroll associated virus (GLRaV) -4 and -6 was continued in 2005. In this effort 4692 and 3832 nucleotides (nt), respectively, for GLRaV-4 and -6 were sequenced. Open reading frames (ORF) identified and the genome organization for each virus included: partial sequence of ORF 1a followed by ORF 1b encoding for a protein recognized as viral RdRp domain. ORF 2 encodes for a small hydrophobic protein followed by ORF 3 encoding for HSP70 homologue protein of 533 aa in length for both viruses. ORF 4 encodes for a putative protein of 539 aa long (P60). ORF 5 encodes for the viral coat protein of estimated molecular mass of 29.5 and 29.2 kDa, and OFR 6, a possible CP minor (CPm) gene, encodes for a putative product of 23.1, and 22.9 kDa, respectively for GLRaV- and -6.
