Grapevine Fanleaf Virus – Detection and Distribution

A uniform source of GFLV from the Golino Virus Collection has been chip-bud inoculated into rooted dormant cuttings of the plants listed in the table below. The plants have callused for 4 to 6 weeks and are now establishing in the greenhouse. By summer 1993 they should be ready for field planting. Plant materials were grafted in the summer of 1991 but these materials are not uniformly infected and it was decided that it would be best to begin again. Green cuttings of Vitis berlandieri and Muscadinia rotundifolia are now rooting and will be green-graft inoculated with GFLV infected wood from the same source as that used in the dormant rooting inoculation. A site on campus has been chosen for the trial and will soon be staked. Reports of findings from this trial will be sent to the AVF after symptoms and ELISA positive reactions are detected.